D9163
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D9163
Sigma
DL -Dithiothreitol
for electrophoresis, ≥99%
| Synonym: | threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT |
| CAS Number: | 3483-12-3 |
| Linear Formula: | HSCH2CH(OH)CH(OH)CH2SH |
| Molecular Weight: | 154.25 |
| Beilstein Registry Number: | 1719757 |
| EC Number: | 222-468-7 |
| MDL number: | MFCD00004877 |
| PubChem Substance ID: | 24894266 |
Description
| Analysis Note | Tested for use in reduced SDS-PAGE |
| Application | An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM). |
Properties
| grade | for electrophoresis |
| assay | ≥99% |
| mp | 40-44 °C |
| 41-44 °C(lit.) | |
| cation traces | heavy metals (as Pb): <10 ppm |
| abs. | A |
| storage temp. | 2-8°C |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 22-36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
| RTECS | EK1610000 |
| Flash Point(F) | 235.4 °F |
| Flash Point(C) | 113 °C |
References
| reference | Cleland, W.W. Biochemistry 3, 480, (1964) Abstract |
| Ruegg, U.T., Cleavage of disulfide bonds in proteins. Meth. Enzymol. 47, 111, (1977) | |
| Sears, D.W., In the investigation of incremental and kinetic reduction in proteins. Biochemistry 16, 2031, (1977) | |
| MacDonald, R.J., Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds. Meth. Enzymol. 152, 219, (1987) | |
| Bacarese-Hamilton, A., Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Ann. N.Y. Acad. Sci. 448, 571, (1985) | |
| Jabusch, J.R., In protein sequence analysis. Anal. Biochem. 91, 532, (1978) | |
| Merck | Merck 13,3412 |
| Beilstein | Beil. 1,III,2360 |
| Aldrich MSDS 1, 840:C / Corp MSDS 1 (1), 1483:A / FT-IR 1 (1), 263:C / FT-IR 2 (1), 417:D / FT-NMR 1 (1), 424:A / IR-Spectra (3), 155:E / IR-Spectra (2), 139:A / NMR-Reference 2 (1), 230:B / RegBook 1 (1), 283:C / Sigma FT-IR 1 (2), 534:A / Structure Index 1, 38:E:8 |
